Case 1
Cindy Stone: She wasn't even a proper murder victim. She was just killed at the spare of the moment.
Case 2
Mia Fey: One of only two female victims with a proper motive against her. She is a strong, powerful woman who was investigating other crimes which landed her in trouble.
Case 3
Jack Hammer: Also not a proper murder, but it easily could have been a woman.
Case 4
Robert Hammond & Gregory Edgeworth: Powerful, great defense attorneys. Of course they are both men because apparently one female defense attorney was enough for the game.
Case 5
Neil Marshall: Apparently one female prosecutor is all they can have too.
Bruce Goodman: A detective striving for truth, etc, who could have been a woman. The only female detective in the game, Angel, is a floozy who serves lunch. LUNCH!
The Darke Victims: 5, of which 2 were women. We don't know much about them apart from one was a boy.

Case 1
Dustin Prince: Probably had to be a guy because he and Maggey were supposed to be lovers, even though that was later revealed as gossip.
Case 2
Dr Grey: Apparently women can't own medical clinics.
Case 3
Mr Berry: Regina was supposed to be the victim, so it's not a murder either. Also, I guess they couldn't kill a teenage girl (but they COULD kill a boy in RFTA).
Case 4
Juan Corrida: One of the few that actually had to be a man, because of his relationship with Celeste.

Case 1
Doug Swallow: He didn't have to be Dollie's boyfriend, and there are plenty of female neuroscientists (or whatever he was doing)! I don't think there are any female geniuses in the series.
Case 2
Kane Bullard: A CEO, so 'of course' it just had to be a man.
Case 3
Glen Elg: Another genius, this time a programmer. ''It couldn't" be a woman because we all know women don't program. Even Lisa Basil wasn't a programmer (but I think she may have been a higher up, at least. )
Case 4
Valerie Hawthorne: The only other proper female murder victim. She is a lieutenant in the police force (the highest rank so far, I think) and a strong motive against her. Seeing as she was a lieutenant, I'm very surprised they didn't make her a man.
Case 5
Misty Fey: She doesn't really count either because the killer was trying to kill someone who was already dead.

Case 1
Shadi Smith: Also probably one of the few that had to be a man (because of Lamiroir).
Case 2
Pal Meraktis: Apparently women can't own medical clinics.
Case 3
Romein LeTouse: A big, tough interpol agent posing as a personal bodyguard. The only big, tough woman in the series was Plum, and she was stuck to sweeping the streets! It would also make more sense if Lamiroir had a female personal bodyguard (to me, anyway).
Case 4
Drew Misham: Not a murder victim.

Apparently ALL SEVEN VICTIMS are male.

Two of them were nurses, who killed their superiors (both of whom were men), one killed in self-defense, and the other was a total Yandere. It does not get much more generic!

How exactly is Drew Misham not a murder victim? Just because he licked a poisoned stamp meant for his daughter, doesn't mean that it isn't murder.

1-3, Jack is going to kill Dee
1-4, Hammond made Yanni act insane.
1-5, Neil had to be able to fight a serial killer (Not being sexist, just the whole gender role thing of girls being dainty)
2-1, he had to be Maggey's lover
2-2, Grey is a jerk
2-3, ringmasters are mainly male. I've never seen a female ringmaster.
2-4, Corrida needed to be with Celeste and Adrian
3-1, Doug had to date Dollie
3-2, Kane was blackmailing Atmey
3-3, Glen had taken out a loan from the Tiger, and spent all his money on gambling
4-1, it had to be Zak
4-2, Pal tried to kill Alita
4-3, Romein had to be a man so he would take the shots (Just saying that in the AA series, woman are never very bulked up)
4-4, Drew stole one of his daughter's prized possessions.
The only exceptions are Bruce and Gregory, who could of been girls but it doesn't matter much.

I'd say the victim of this case counts as well, as the one Godot was trying to kill was a woman.

1-3: Jack Hammer had to be a male action star and a rival of sorts to Will Powers.
1-4: if Hammond were a woman, Yanni Yogi couldn't have impersonated the victim.
1-5: same thing. If the victim were a woman, Jake Marshall couldn't have impersonated her. Also, among SL-9 detectives, there were already two of the feminist icons in the game: Lana Skye and Angel Starr.
2-1: Maggey Byrde's lover. If the victim were a woman, I'm sure Gumshoe wouldn't be too happy about it.
2-2: both the defendant and the culprit are women already.
2-3: the fact that Russell Berry is a father figure is important to the plot.
2-4: same as 1-3. Also, the love triangle doesn't make sense if Juan Corrida's a woman.
3-1: love triangle.
3-2: corrupt CEO. Not exactly a good image for a woman to have... but yeah, I suppose Kane Bullard could've been Kate Bullard.
3-3: yep, I suppose Glen Elg could've been a woman. As for Lisa Basil, I'm pretty sure she's the CEO of Blue Screens.
4-1: Zak Gramarye has to be a man for so many reasons I can't even list them.
4-2: same as 3-2.
4-3: I see your point, but the victim has to be big and tough in order not to die instantly from the bullet.

"1-3: Jack Hammer had to be a male action star and a rival of sorts to Will Powers."
The Evil Magistrate could have been a woman... besides, I didn't see much rivalry between them.
"1-4: if Hammond were a woman, Yanni Yogi couldn't have impersonated the victim."
True, but Yanni could have been a girl. Like Lind says, there were hardly any female killers.
"1-5: same thing. If the victim were a woman, Jake Marshall couldn't have impersonated her. Also, among SL-9 detectives, there were already two of the feminist icons in the game: Lana Skye and Angel Starr."
Feminist Icons? I really doubt a... floozy like Angel Starr who has about 5 boyfriends and wears such revealing clothes is a feminist icon. (Still, she is one of the funniest characters in the game.) I wouldn't exactly call Lana an 'icon' either.
"2-1: Maggey Byrde's lover. If the victim were a woman, I'm sure Gumshoe wouldn't be too happy about it."
They weren't lovers. Maggey herself said she bought the present after he covered one of her shifts, that's all. There might have been a bit of chemistry there, but judging by how she acted extremely happy in the trial I would doubt they were close.
"2-2: both the defendant and the culprit are women already."
Umm, how many cases are there that the defendant, culprit and the victim are men?!
"2-3: the fact that Russell Berry is a father figure is important to the plot."
...Mother figure?
"2-4: same as 1-3. Also, the love triangle doesn't make sense if Juan Corrida's a woman."
Yeah, I agree with that one.
"3-1: love triangle."
There wasn't a love triangle. The only reason Feenie pushed Doug was because he was talking badly about Dahlia. He would have done it if it were a girl. And Dahlia would have had access to the lab if it was her one of her female friends.
"3-2: corrupt CEO. Not exactly a good image for a woman to have... but yeah, I suppose Kane Bullard could've been Kate Bullard."
We need all types of people, male and female. You could say the same about a man.
"3-3: yep, I suppose Glen Elg could've been a woman. As for Lisa Basil, I'm pretty sure she's the CEO of Blue Screens."
Well, at least we know that the series CAN have female CEOs.
"4-1: Zak Gramarye has to be a man for so many reasons I can't even list them."
I could only think of two or three. But anyway.
"4-2: same as 3-2."
"4-3: I see your point, but the victim has to be big and tough in order not to die instantly from the bullet."
And we all know women can't be big and tough, right?

Doesn't Misty Fey technically count? Regardless of intended target, the victim would have to be a woman in this case, because only spirit mediums can be women. As Godot pointed out, the victim could have been Pearl, since she was also trying to summon Dahlia's spirit that night. Furthermore, had Godot witnessed Maya channeling Dahlia in order to protect herself, there's that small chance she could have been a victim as well.

1-1: Cindy was sorta cheating on Larry, although that wasn't the reason she was killed
1-3: Jack tried to kill Dee Vasquez and was planning on pinning it on Will
1-4: Robert didn't believe in his clients, which led to one of his clients killing him
2-4: Juan forged a suicide note and was about to expose it, for the purpose of ruining Matt's career
3-2: Bullard was like Redd. He blackmailed Atmey, too.
3-3: Glen was a hacker, and about to let that virus loose.
3-4: Valerie got Terry for a murder that didn't actually happen
4-1: Shadi was trying to cheat in poker and ruin Phoenix's name.
4-2: He almost murdered Alita, and didn't mention the bullet in Wocky's heart and acted as if everything was okay
4-4: Because of Drew's forging business, Phoenix lost his attorneys badge